Application

Research CategoryProtein Trafficking

Immunohistochemistry: 1:1,000-1:5,000. Tissue fixed in 10% formalin, Bouin′s fixative for 1-2 days and embedded in paraffin, or unfixed frozen tissues may be used.

Immunoblotting: 1:1,000-1:5,000.

Optimal working dilutions must be determined by end user.

Anti-Ubiquitin Antibody detects level of Ubiquitin & has been published & validated for use in WB, IH, IH(P).

Research Sub CategoryUbiquitin & Ubiquitin Metabolism

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Immunogen

Purified uboquitin covalently coupled to KLH using glutaraldehde.

Legal Information

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

Physical form

Neat rabbit antisera with 10 mM sodium azide.

Specificity

Ubiquitin (76 amino acid, 8.5 kDa). Shows staining of neurofibrillary tangles, neurites in senile plaques and a subset of neuronal processes. No reactivity seen to tau, neurofilament or microtubule-associated proteins.

Storage and Stability

Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles.